Grabda J., 1983. Studies on viability and infectivity of Anisakis simplex stage III larvae in fresh salted and spiced Baltic herring. Acta Ichthyol. Piscat. 13 (2): 117-129.
The studies involved periodical tests of salted and spiced Baltic herring for the presence of live Anisakis simplex larvae through inducing their spontaneous movements when kept in a liver extract-fresh bovine blood medium in a thermostat set at 37°C for 4 days. The infectivity of the larvae was studies by in vitro observing their development in a medium such as above. A period of time necessary to kill all the Anisakis larvae present in salted an spiced herring was determined.